The antioxidant trolox inhibits aging and enhances prostaglandin E-2 secretion in mesenchymal stem cells

Mesenchymal stem cells (MSCs) have been widely used in regenerative medicine and clinical therapy due to their capabilities of proliferation, differentiation, immune regulation. However, during vitro expansion, MSCs are prone aging, which largely limits application. Prostaglandin E-2 (PGE-2) is a key effector secreted by exert immunomodulatory effects. By screening the compound library for PGE-2 secretion, antioxidant trolox was verified as stimulator secrete PGE-2. The effect on biological characteristics MSCS, including gene expression, examined. results demonstrated that can resist promote enhance secretion without affecting surface marker expression. Furthermore, treatment up-regulates miR-17-92 clusters may contribute its anti-aging Thus, addition might be beneficial expansion